Structural elucidation and immunological activity of a polysaccharide from the fruiting body of Armillaria mellea |
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| Authors: | Yongxu Sun Haitao Liang Xiantao Zhang Haibin Tong Jicheng Liu |
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| Affiliation: | 1. Department of Pharmacology, Qiqihar Medical University, Qiqihar 161042, China;2. Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;3. New Medicine Development Limited Company, Jilin Revision Pharmaceutical Industry, Changchun 130012, China;4. Life Science Research Center, Beihua University, Jilin 132013, China;1. Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan, ROC;2. Department of Radiation Oncology, Mackay Memorial Hospital, Taipei, Taiwan, ROC;3. Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC;4. Institute of Pharmacology, Taipei Medical University, Taipei, Taiwan, ROC;5. Department of Radiation Oncology, Taipei Medical University-Wan Fang Hospital, Taipei, Taiwan, ROC;6. Department of Biotechnology, HungKuang University, Taichung, Taiwan, ROC;7. Department of Bioscience Technology, College of Health Science, Chang Jung Christian University, Tainan, Taiwan, ROC;8. Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC;1. Department of Urology, Fuzhou General Hospital, Fuzhou 350025, China;2. Department of Blood Purification, Fuzhou General Hospital, Fuzhou 350025, China;1. Fungal Genomics and Bioinformatics Group, Research Center for Forestry and Wood Industry, University of Sopron, Sopron, 9400 Hungary;2. Department of Biology, University of Toronto, Mississauga, ON L5L 1C6 Canada;3. Synthetic and Systems Biology Unit, Biological Research Center, Hungarian Academy of Sciences, Szeged, 6726, Hungary |
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| Abstract: | ![]()
The water-soluble polysaccharide (AMP), with a molecular mass of 7.8 × 103 Da as determined by high-performance size-exclusion chromatography (HPSEC), was obtained from the fruiting body of Armillaria mellea. Methylation, Smith degradation, acetolysis, 1H and 13C NMR spectroscopy and acid hydrolysis studies were conducted to elucidate its structure. The results indicated that AMP consisted of a backbone composed of (1→6)-linked-α-d-glucopyranosyl, (1→2,6)-linked-α-d-glucopyranosyl and (1→6)-linked-α-d-galactopyranosyl residues in the ratio of 3:1:1, and terminated with one single terminal (1→)-β-d-glucopyranosyl at the O-2 position of (1→2,6)-linked-α-d-glucopyranosyl, on average, along the main chain. Preliminary tests in vitro showed that AMP has stimulating effects on murine lymphocyte proliferation induced by concanavalin A or lipopolysaccharide in a dose-dependent manner. It is a possible potential immunopotentiating agent for use in health-care food or medicine. |
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