Genetic analysis of protoplast fusant Xhhh constructed for pharmaceutical wastewater treatment |
| |
| Authors: | Xu-Xiang Zhang Hai-Ying Jia Bing Wu Da-Yong Zhao Wei-Xin Li Shu-Pei Cheng |
| |
| Affiliation: | 1. State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, 22, Hankou Road, Nanjing University, Nanjing, Jiangsu 210093, China;2. Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing, 210042, China;1. Department of Chemistry & Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India;2. Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur 721302, West Bengal, India;3. Division of Plant Biology, Bose Institute, P-1/12, C.I.T. Scheme VII M, Kolkata 700054, West Bengal, India;1. Division of Chemistry, Faculty of Science and Humanities, Sree Sowdambika College of Engineering, Chettikurichi, Aruppukottai 626 134, Virudhunagar District, Tamil Nadu, India;2. Department of Chemistry, Devanga Arts College (Autonomous), Aruppukottai 626 101, Virudhunagar District, Tamil Nadu, India;3. Department of Chemistry, Pioneer Kumaraswamy College, Vetoornimadam, Nagercoil 629 003, Kanyakumari District, Tamil Nadu, India;1. Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan;2. Research Unit for the Physiological Chemistry, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan;3. Institute of Technology and Science, The University of Tokushima, 2-1 Minami-josanjima, Tokushima 770-8506, Japan;4. Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo-otani, Sogabe-cho, Kameoka, Kyoto 621-8555, Japan;1. Department of Psychiatry, Psychosomatic Medicine and Psychotherapy, University Hospital Frankfurt, Frankfurt am Main, Germany;2. Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital of Würzburg, Germany;3. Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria;4. Department of Neuropsychopharmacology, Institute of Psychology, University of Tartu, Tartu, Estonia;1. Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico;2. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, 62250 Cuernavaca Mor, Mexico;1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China;2. Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, PR China |
| |
| Abstract: | ![]()
In order to analyse genetic relationships between functional strain Xhhh previously constructed through protoplast fusion for pharmaceutical wastewater treatment and its parents, random amplification polymorphic DNA (RAPD) and polymerase chain reaction (PCR) were used to investigate genetic similarities among the strains based on genome and functional genes analyses. A total of 739 clear and consistent bands were produced in the RAPD fingerprint analysis with 40 primers. The genetic similarity indices between Xhhh and parental strains PC (Phanerochaete chrysosporium), SC (Saccharomyces cerevisiae) and XZ (native bacterium Bacillus sp.) were 36.21%, 37.73% and 37.48%, respectively. With PCR amplification and DNA sequencing, Xhhh was found containing functional genes of mnp and lip from PC, FLO1 from SC and 16S rDNA fragments from XZ. Experimental results of genetic analyses were in accordance with Xhhh biochemical and phenotypic characteristics, and protoplast fusion technique is considered as a promising technique in environmental pollution control. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
