<Emphasis Type="Italic">In vitro</Emphasis> selection of salt tolerant cell lines in <Emphasis Type="Italic">Solanum tuberosum</Emphasis> L. |
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Authors: | F Queirós F Fidalgo I Santos R Salema |
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Institution: | (1) Departamento de Botanica, Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal;(2) Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal |
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Abstract: | Cell lines able to grow on media containing 50, 100, 150 or 200 mM NaCl were established from potato callus cultures by direct
recurrent selection or gradual selection. In callus subjected to direct selection only small clusters of cells survived on
medium with 150 or 200 mM NaCl, whereas on 100 mM small cell portions appear necrotic. When cell lines were obtained by successive
subcultures on media with increased concentrations of NaCl, salt-tolerant calli were more compact and developed a greenish
colour free from necrotic areas. The response of calli lines grown on media with NaCl was compared to control line. The NaCl-tolerant
calli showed a decrease in relative growth rate and water content, with higher reductions in the 150 mM tolerant callus. Lipid
peroxidation was increased in 50 mM and 100 mM NaCl-tolerant calli, while in 150 mM tolerant callus remained similar to 100
mM values. There was a significant increase in ascorbic acid content in 100 mM and 150 mM NaCl-tolerant calli as compared
to the 50 mM, that was two-fold the value found in the control. Also, the contents of soluble and insoluble proteins increased
in salt-tolerant lines. SDS-PAGE of soluble proteins showed the synthesis of specific polypeptides in the presence of NaCl
in culture medium and the synthesis of a new polypeptide. |
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Keywords: | ascorbic acid callus tissue lipid peroxidation NaCl tolerance potato proteins salt stress |
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