Identification and characterization of a xyloglucan-specific family 74 glycosyl hydrolase from Streptomyces coelicolor A3(2) |
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| Authors: | Enkhbaatar Bolormaa Temuujin Uyangaa Lim Ju-Hyeon Chi Won-Jae Chang Yong-Keun Hong Soon-Kwang |
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| Institution: | Division of Bioscience and Bioinformatics, Myongji University, Yongin, South Korea. |
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| Abstract: | The sco6545 gene of Streptomyces coelicolor A3(2) was nominated as a putative cellulase with 863 mature-form amino acids (90.58 kDa). We overexpressed and purified Sco6545 and demonstrated that the protein is not a cellulase but a xyloglucan-specific glycosyl hydrolase which cleaves xyloglucan at unbranched glucose residues. |
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