NGX6基因在多种常见癌组织中的原位表达及其临床意义的研究

研究新的候选抑瘤基因NGX6在多种常见癌组织中的mRNA原位表达谱,分析NGX6 mRNA阳性率与肿瘤1临床病理特征的关系并评估其作为肿瘤转移、预后预测分子标志物的有效性.利用已制作的多肿瘤组织和鼻咽癌组织微阵列,原位杂交检测NGX6 mRNA在多种常见癌组织中的阳性率.结果显示.NGX6 mRNA在鼻咽癌、肺癌、胃癌和结、直肠癌中的阳性率低于其对应的正常组织(P＜0.05,P＜0.01).淋巴结转移性鼻咽癌、肺癌、结、直肠癌和喉癌组织中的NGX6 mR.NA阳性率显著降低(P＜0.05,P＜0.01).NGX6 mRNA阳性率与鼻咽癌、肺癌和结、直肠癌临床分期有关,临床Ⅱ期、Ⅲ期或Ⅳ期癌组织NGX6 mRNA阳性率明显低于其相应的临床Ⅰ期癌(P＜0.05,P＜0.01).研究表明,鼻咽癌、肺癌、胃癌和结、直肠癌中存在低水平的NGX6 mRNA,NGX6 mRNA可作为鼻咽癌、肺癌和结、直肠癌侵袭、转移和临床进展预测的分子标志.

Study of In situ Expression of NGX6 Gene in The Several Common Types of Cancer and Its Clinical Significance

To investigate the mRNA expression profile of novel candidate of tumor suppressor gene NGX6 in the several common types of cancer, analyze the correlation between NGX6 mRNA expression and its clinicopathological and to evaluate the validity that NGX6 mRNA act as molecular marker for tumor metastasis and prognosis. Multi-tumor tissue and nasopharyngeal cancer tissue microarrays were constructed previously and tissue microarrays combined with in situ hybridization were used to detect the expression of NGX6 mRNA in the human several common types of cancer. Results showed that expression of NGX6 mRNA in the nasopharyngeal cancer (NPC), lung cancer, gastric cancer and colon-rectal cancer was significantly lower than that in their non-cancer normal tissue (P < 0.05, P < 0.01). Expression level of NGX6 mRNA was evidently lower in the NPC, laryngeal cancer, lung caner and colon-rectal cancer with lymph node metastasis (P < 0.05, P < 0.01). Expression of NGX6 mRNA in the NPC, lung caner and colon-rectal cancer had significant correlation with their clinical stages, which NGX6 mRNA was significantly lower in the clinical stage T2, T3 or T4 that than their clinical stage T1(P < 0.05, P < 0.01). The results suggested that the NPC, lung caner, gastric cancer and colon-rectal cancer had evidently lower expression of NGX6 mRNA, NGX6 mRNA might be used as molecular markers for invasion, metastasis and prognosis of NPC, lung caner and colon-rectal cancer.