Kinetic study on starch hydrolysis using a glucose/maltose sensing system |
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| Authors: | Hong-Bo Qu Xian-En Zhang Wen-Wu Chui Shu-Zheng Zhang Gao-Xiang Li Fan Ouyang |
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| Institution: | (1) Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, P.R. China;(2) Present address: National Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080 Beijing, P. R. China;(3) Present address: Chinese Medical College of Hubei, 430070 Wuhan, P.R. China;(4) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, P. R. China;(5) National Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080 Beijing, P. R. China |
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| Abstract: | Summary A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10  L sample size with recycled back-flow minimises any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems. |
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