A simple and established method of tissue culture of human gingival fibroblasts for gingival augmentation |
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| Authors: | Saczko Jolanta Dominiak Marzena Kulbacka Julita Chwiłkowska Agnieszka Krawczykowska Honorata |
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| Affiliation: | Department of Medical Biochemistry, Wroclaw Medical University, Wroc?aw, Poland. michal@bioch.am.wroc.pl |
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| Abstract: | Recent advances in tissue engineering technology suggest its application in different medical fields, including periodontology. There are some reports of new non-enzymatic methods of isolating human gingival fibroblast for short-time cultivation in vitro to be used in autologous gingival augmentation. The aim of this study was to obtain a simple and established method of culturing human gingival fibroblasts. The authors developed a recurrent method that can be successfully used in autologous gingival augmentation. |
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