AFP蛋白芯片技术的检测流程优化

目的:通过优化现有的蛋白芯片检测过程,在保证检测准确性的同时缩短甲胎蛋白(Alpha Fetal protein,AFP)的检测时间,提高检测效率,为原发性肝癌的筛查提供经济、便捷、省时、有效的检测方法。方法:本研究在传统蛋白芯片检测流程(1-1.5小时)的基础上,通过优化检测流程将检测时间缩短至18分钟,并且通过和传统方法进行比较,评价该优化方法的检测效能。结果:与传统蛋白芯片检测方法相比,本优化方法的检测时间缩短至18分钟。重复检测同一样本,传统方法 AFP水平为16.50±1.172ng/m L,优化方法 AFP水平为18.33±1.029 ng/m L,结果无明显统计学差异(P=0.251)。结论:本研究成功地优化了AFP的蛋白芯片检测流程,在缩短检测时间的同时,保证了检测的准确率,是一种经济省时易操作的AFP检测方法。

Optimization of Protein Microarray Procedure for Detecting Alpha-fetoprotein

ABSTRACT Objective: To optimize the traditional protein microarray procedure for detecting AFP (Alpha Fetal protein) by short- ening the detection time, providing an effective, economical, easily performed and time-saving method for screening primary liver cancer. Methods: The optimized procedure significantly shortened the detection time to 18 minutes on the basis of traditional protein microarray detection procedure (1-1.5 hours). Serum sample was examined using improved and traditional procedure of protein microarray to verify the accuracy of improved procedure. Results: Compared with the traditional procedure of protein microarray, the improved procedure shortened the detection time to 18minutes. The results of repetition test of the same sample indicated that no statistically significant dif- ference was found in the AFP levels between traditional procedure and improved procedure(16.50±1.172 ng/mL vs 18.33±1.029 ng/mL, p=0.251). Conclusion: On the premise of ensuring detection accuracy, this study successfully optimized the AFP protein microar- ray detection procedure by shortening the detection time.