骨髓单个核细胞移植对大鼠溃疡性结肠炎的作用

目的:探讨同种异体骨髓单个核细胞(BM-MNCs)移植大鼠溃疡性结肠炎模型的作用。方法:将DAPI标记的同种异体大鼠骨髓单个核细胞(BM-MNCs)经尾静脉注射移植到大鼠溃疡性结肠炎(UC)模型体内(模型组),以尾静脉注射等量PBS的UC大鼠作为对照组。光镜观察大鼠结肠组织病变改变,荧光显微镜观察标记DAPI的BM-MNCs在结肠组织中的定植及分布情况,免疫荧光检测BM-MNCs中CK19、CD34的表达情况。结果:移植组大鼠结肠组织可见新生黏膜上皮及腺体,黏膜下有新鲜至成熟肉芽组织生成,明显优于对照组;移植14天,大鼠结肠组织中可观察到DAPI标记的BM-MNCs细胞;DAPI标记的细胞可表达血管内皮细胞特异性表达蛋白CD34或黏膜上皮细胞特异性表达蛋白CK19。结论:BM-MNCs可向受损病变部位结肠组织迁移和定植,且分化为血管内皮细胞和黏膜上皮细胞。

Effect of BM-MNCs Transplantion on the Ulcerative Colitis in Rats

ABSTRACT Objective: To explore the effect bone marrow mononuclear cells(BM-MNCs) transplantion on the ulcerative colitis in rats. Methods: BM-MNCs labeled with DAPI were transplanted into UC rat (model group) through caudal vein, the rats injected with the same amount of PBS were used as the control group. The pathological changes of colon tissues was observed by light microscope; and the planting and distributed conditions of the BM-MNCs labeled DAPI in colon tissues were detected by fluorescence microscope, the expressions of CK19 and CD34 in BM-MNCs were detected by immunofluorescence. Results: The neogenetic mucosal epithelium and gland submucous neogenetic and ripe granulation tissue were observed in the transplanting group, which were obviously better than those of the control group; DAPI labeled BM-MNCs cells can express CD34 and CK19. Conclusion: BM-MNCs can transfer and plant into the injured colon tissues and differentiate into the vascular endothelial cell and mucosal epithelial cell.