shPLCε通过下调CDC25A抑制T24细胞的瓦伯格效应

目的:探讨shPLCε对膀胱癌T24细胞瓦伯格效应的影响及其相关机制。方法:(1)慢病毒感染T24细胞,葡萄糖测定试剂盒和乳酸测试盒分别检测细胞葡萄糖利用和乳酸生成情况;q-PCR、Western blot分别检测PLCε、CDC25A及瓦伯格效应相关分子的表达。(2)转染sh CDC25A质粒,q-PCR、Western blot检测CDC25A的表达;Western blot检测瓦伯格效应相关分子的表达情况。结果:(1)慢病毒干扰PLCε后,T24细胞利用葡萄糖和生成乳酸的能力降低,同时下调CDC25A、PKM2、GLUT1、LDHA的表达。(2)干扰CDC25A的表达后可抑制PKM2、GLUT1、LDHA的表达。结论:shPLCε通过下调关键分子CDC25A的表达抑制膀胱癌T24细胞的瓦伯格效应,从而为膀胱癌的治疗提供了新思路。

Effects of shPLCε on Warburg Effect Through CDC25A in T24 Cells

Objective:To study the effect of shPLCε on Warburg effect in bladder cancer and the potential mechanism.Methods:(1) Using lentivirus infecting T24 cells,the glucose consumption and lactate production were detected by the corresponding kit. The expression of PLCε、CDC25A and the molecules related with Warburg effect such as PKM2, GLUT1, LDHA were detected by q-PCR and Western blot. (2)To investigate the related molecular mechanism, using plasimid infecting T24 cells, the expression of CDC25A was detected by q-PCR and Western blot. The expression of molecules related with Warburg effect were detected by Western blot.Result:(1)After reducing the expression of PLCε, glucose consumption and lactate production were significantly decreased in the LV-sh PLCε group,compared with the blank control group and LV-NC group (P<0.01).q-PCR and Western blot showed that the expression of CDC25A, PKM2, GLUT1, LDHA were remarkably lower in LV-shPLCε group than that in blank group and LV-NC group (P<0.05). (2)Western blot showed that the expression of PKM2, GLUT1, LDHA were remarkably lower in shCDC25A group than in blank group and shNC group(P<0.05), after treated with shCDC25A-plasimid.Conclusion:shPLCε can reduce Warburg effect through decreasing the expression of CDC25A in bladder cancer, which can not provide energy for the development and progression of bladder cancer.