骨形成蛋白9对人膀胱癌BIU-87细胞增殖和迁移的影响

目的:人骨形成蛋白9(bone morphogenetic protein 9,BMP9)对人膀胱癌BIU-87细胞增殖和迁移的影响。方法:使用过表达BMP9基因的腺病毒(AdBMP9)感染BIU-87细胞,采用定量PCR检测BMP9 mRNA的表达,Western blot检测BMP9蛋白及BMP9下游相关信号通路蛋白的表达;MTT及集落形成实验检测BIU-87细胞增殖能力;划痕愈合实验及Transwell ^TM小室迁移实验检测BIU-87细胞迁移能力。结果:感染AdBMP9后,BIU-87细胞中BMP9的mRNA水平和蛋白质水平均显著增加;过表达BMP9后,BIU-87细胞的体外增殖和迁移能力明显增加;Western blot结果显示BMP9可明显激活AKT信号通路。结论:高表达BMP9可能通过激活AKT信号通路促进人膀胱癌BIU-87细胞的增殖和迁移。

The Effects of Bone Morphogenetic Protein 9(BMP9) on the Proliferation and Migration of Human Bladder Cancer BIU-87 Cells

Objective:To investigate the effects of bone morphogenetic protein 9 (BMP9) on the proliferation and migration of human bladder cancer BIU-87 cells.Methods:BIU-87 cells were infected with BMP9 recombinant adenovirus(AdBMP9); The mRNA levels of BMP9 were detected by Q-PCR; The BMP9 protein and its downstream associated signaling pathway protein levels were detected by Western blot; The MTT and colony formation assay were used to test the proliferation of BIU-87 cells; The wound healing and Transwell ^TM chamber assay were taken to evaluate the migration of BIU-87 cells. Results:The expression of BMP9 in BIU-87 cells was highly increased after AdBMP9 infection; Forced expression of BMP9 promotes both the proliferation and migration of BIU-87 cells; Western blot results showed that BMP9 overexpression also increased AKT phosphorylation.Conclusion:High levels of BMP9 may promote the proliferation and migration of bladder cancer BIU-87 cells by activating the AKT signaling pathway.