Nesfatin-1及MCH对大鼠胃酸分泌和胃运动的影响

目的:本实验主要探究nesfatin-1对胃运动和胃酸分泌的影响,以及弓状核(ARC)-下丘脑外侧区(LHA)nesfatin-1神经通路在该过程中的作用。方法:采用逆行追踪和免疫组织化学染色实验观察ARC-LHA nesfatin-1神经通路的构成;在体胃运动实验观察nesfatin-1对胃运动的影响以电刺激ARC对胃运动的影响;采用幽门结扎法测量胃液和胃酸分泌量。结果:LHA微量注射nesfatin-1抑制胃运动和胃酸分泌,但是预先注射黑色素浓集激素(MCH)受体拮抗剂PMC-3881-PI减弱nesfatin-1对胃运动和胃酸分泌的抑制作用。电刺激ARC后,胃收缩幅度和频率显著增强,胃酸分泌明显增多。nesfatin-1抗体或PMC-3881-PI对电刺激ARC诱导的胃运动没有显著影响,但是能够改变电刺激ARC诱导的胃酸分泌。结论:ARC-LHA间nesfafin-1通路可调控大鼠胃运动和胃酸分泌,并且黑色素浓集激素也参与调节该过程。

The Effect of Nefatin-1 and MCH on Gastric Motility and Gastric Acid Secretion in Rats

ABSTRACT Objective: The aim of this study was to investigate the effects of nesfatin-1 on gastric motility and gastric acid secretion and the role of nesfatin-1 neural pathway in the arcuate nucleus (ARC) -the lateral hypothalamic area (LHA) in this process. Methods: The retrograde tracing and immunohistochemical staining was used to observe the projection of nesfatin-1 nerve fibers. The effect of nesfatin-1 or electric stimulation of the Arc on the amplitude and frequency of gastric contraction was observed by Gastric motility recording in vivo. Pylorus ligation was used to measure gastric secretion volume and acid output. Results: Administration of nesfatin-1 into LHA could inhibite gastric motility and gastric acid secretion. however pretreatment with PMC-3881-PI could attenuate the inhibitory effect of nesfatin-1 on gastric motility and gastric acid secretion. Electrical stimulation of Arc promoted the gastric motility and gastric secretion Nesfatin-1 antibody or PMC-3881-PI had no significant effect on Arc stimulation-induced gastric motility, but was able to alter Arc stimulation-induced gastric acid secretion. Conclusion: The nesfafin-1 pathway between ARC-LHA regulates gastric motility and gastric acid secretion in rats, and melanin-concentrating hormone is also involved in regulating this process.