夏枯草胶囊对自身免疫性甲状腺炎大鼠甲状腺组织及Fas、FasL表达的影响

目的:探讨夏枯草胶囊对自身免疫性甲状腺炎(AITD)大鼠甲状腺组织病理学及Fas、FasL表达的影响。方法:将40只雌性SD大鼠按照随机数表法随机分为空白对照组、模型组、夏枯草胶囊组、硒酵母组,每组10只,空白对照组予以正常饲养,模型组和药物组予以PTg皮下注射建立AITD模型,夏枯草胶囊组、硒酵母组分别给予夏枯草胶囊与硒酵母片的生理水溶液灌胃,药物干预6周后处死大鼠,取甲状腺组织,HE染色电镜下观察各组大鼠甲状腺组织形态,免疫组化法检测各组大鼠甲状腺组织Fas、FasL蛋白的表达。结果:1)模型组大鼠甲状腺组织滤泡大量破坏,形态不规则,胶质分布不均匀,滤泡间隙增大,可见大量淋巴细胞及浆细胞浸润。夏枯草胶囊组滤泡形态尚规则,胶质含量较空白组少,滤泡间有少量淋巴细胞浸润。与模型组对比,夏枯草胶囊组与硒酵母组滤泡破坏及淋巴细胞浸润有显著改善。2)对照组大鼠甲状腺组织中Fas、FasL蛋白仅呈少量表达,模型组大鼠甲状腺组织中Fas、FasL蛋白表达较对照组显著增加(P0.01),夏枯草胶囊组大鼠甲状腺组织中Fas、FasL蛋白表达较模型组及硒酵母组均显著减少(P0.01)。结论:夏枯草胶囊可能通过减少Fas、FasL的表达,抑制甲状腺滤泡细胞凋亡,从而减轻甲状腺滤泡上皮的破坏。

Effect of Prunella Vulgaris Capsule on Thyroid Tissue and Expression of Fas /FasL in Rats with Autoimmune Thyroiditis

ABSTRACT Objective: To study and observe the effect of prunella vulgaris capsule on the thyroid tissue and expression of Fas /FasL in rats with autoimmune thyroiditis. Methods: Forty female SD rats were divided into the control group, AITD model group, Prunella vulgaris capsule group and selenium yeast group. The AITD model was made by subcutaneous injection of PTg. Prunella vul- garis capsule group and selenium yeast group were treated with Prunella vulgaris and selenium yeast. The rats were sacrificed at 6 weeks after the drug intervention. The thyroid tissue was observed by HE staining. The expression of Fas and FasL protein in the thyroid tissue of the rats were observed by immunohistochemistry. Results: 1) The thyroid tissue follicles were greatly destroyed in the model group with irregular shape, uneven distribution of glial, increased follicular gap, and a large number of lymphocytes and plasma cell infiltration. The follicular morphology in Prunella vulgaris capsule group was still regular, and the glial content was lower than that of the control group, there was a small number of lymphocyte infiltration between the follicles. Prunella vulgaris capsule and selenium yeast group showed significant improvement in the follicular destruction and lymphocyte infiltration. 2) In the control group, Fas and FasL protein in thyroid tissue only expressed a small amount; the expressions of Fas and FasL protein in thyroid tissue of model group was significantly higher than that of the control group (P<0.01); the expressions of Fas and FasL protein in thyroid tissue of Prunella vulgaris capsule group were significantly lower than those of the model group and selenium yeast group (P<0.01). Conclusion: Prunella vulgaris capsules may reduce the damage of thyroid follicular epithelium by reducing the expression of Fas and FasL and inhibiting the apoptosis of thyroid fol- licle cells.