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A 570-kb DNA Sequence of the Escherichia coli K-12 Genome Corresponding to the 28.0-40.1 min Region on the Linkage Map |
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| Authors: | Aiba Hiroji; Baba Tomoya; Hayashi Kouji; Inada Toshifumi; Isono Katumi; Itoh Takeshi; Kasai Hiroaki; Kashimoto Kaoru; Kimura Shigenobu; Kitakawa Madoka; Kitagawa Masanari; Makino Kozo; Miki Takeyoshi; Mizobuchi Kiyoshi; Mori Hirotada; Mori Tomoko; Motomura Kouji; Nakade Shinsuke; Nakamura Yoshikazu; Nashimoto Hiroko; Nishio Yoshitaka; Oshima Taku; Saito Noriko; Sampei Gen-ichi; Seki Yasushi; Sivasundaram Suharnan; Tagami Hideaki; Takeda Jun-ichi; Takemoto Keiko; Takeuchi Yasushi; Wada Chieko; Yamamoto Yoshihiro; Horiuchi Takashi |
| Institution: | 1Department of Molecular Biology, School of Science, Nagoya University Furo-cho, Chikusaku, Nagoya 464-01, Japan 2Department of Biology, Faculty of Science, Kobe University 1-1 Rokkodai-cho, Nadaku, Kobe 657, Japan 3National Institute for Basic Biology 38 Nishigonaka, Myodaiji-cho, Okazaki 444, Japan 4Research and Education Center for Genetic Information, Nara Institute of Science and Technology 8916-5 Takayama-cho, Ikoma 630-01, Japan 5Faculty of Pharmaceutical Sciences, Kyushu University 3-1-1 Maedashi, Higashiku, Fukuoka 812-82, Japan 6Research Institute for Microbial Diseases, Osaka University 3-1 Yamadagaoka, Suita 565, Japan 7Department of Applied Physics and Chemistry, University of Electro-Communications 1-5-1 Chofugaoka, Chofu 182, Japan 8Institute of Medical Science, University of Tokyo 4-6-1 Shiroganedai, Minatoku, Tokyo 108, Japan 9Department of BioSciences, Teikyo University 1-1 Toyosatodai, Utsunomiya 320, Japan 10Institute for Virus Research, Kyoto University Kawahara-cho, Shogoin, Sakyoku, Kyoto 606-01, Japan 11Department of Genetics, Hyogo College of Medicine 1-1 Mukogawa-cho, Nishinomiya 663, Japan |
| Abstract: | The 569,750 base pair sequence corresponding to the 28.0–40.1min region on the genetic map of Escherichia coli K-12 (W3110)was determined. This region includes the replication terminusregion and contained at least 549 potential open reading frames.Among them, 160 (29%) were previously reported, 174 (32%) werehomologous to other known genes, 102 (18%) were identical orsimilar to hypothetical genes registered in databases, and theremaining 113 (21%) did not show a significant similarity toany other gene. Of interest was the finding of a large numberof genes and gene clusters in andnear the replication terminationregion which had been thought to be genetically silent. Thoseincludeda cluster of genes for fatty acid ß-oxidation,the third copy of the pot (spermidine/putrescine transport system)gene cluster, the second dpp (dipeptide transport system) operon,the second dsm (anaerobic dimethyl sulfoxide reductase) operon,a cluster of fim (fimbrial) genes anda DNA helicase-like genewith a high molecular weight. In addition, we found the dnaC-and dnaT-like genes in the cryptic prophage, Rac, anda numberof genes originated probably from plasmids. |
| Keywords: | Escherichia coli K12 genome sequence ORF analysis replication termination region |
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