The urine from patients with peritonitis as a rich source for purifying human acid sphingomyelinase and other lysosomal enzymes |
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| Authors: | L E Quintern T S Zenk K Sandhoff |
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| Affiliation: | Institut für Organische Chemie und Biochemie, Universit?t Bonn, F.R.G. |
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| Abstract: | Milligram amounts of human acid sphingomyelinase (EC 3.1.4.12) were purified to 95% homogeneity using urine from patients with acute peritonitis. The activity of this enzyme is elevated more than 200-times in the urine of these patients. To a lesser extent, levels of some other lysosomal hydrolases are also elevated. |
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