| The Role of dinB in the Appearing of Antibiotics Resistance in E.coli |
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| 作者姓名： | XI Yun HUANG Jing TAO Ling ZHAO Peng CHEN You-ming FU Yong-mei SUN Heng-biao YOU Xu XIAO Gang |
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| 作者单位： | Dept. of Lab., 3rd Affil. Hosp. of Zhongshan Uni.,Dept. of Lab., 3rd Affil. Hosp. of S. Med. Uni.,Dept. of Parm,3rd Affil.Hosp.of Zhongshan Uni,Dept. of Lab., 3rd Affil. Hosp. of S. Med. Uni.,Dept. of Lab., 3rd Affil. Hosp. of S. Med. Uni.,Dept. of Emergency, 3rd Affil. Hosp. of Zhongshan Uni., Guangzhou 510630,Dept. of Lab., 3rd Affil. Hosp. of S. Med. Uni.,Dept. of Lab., 3rd Affil. Hosp. of S. Med. Uni.,Dept. of Lab., 3rd Affil. Hosp. of S. Med. Uni. |
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| 基金项目： | 广东省科技计划社会发展项目（2009B030803035）；广州市科技计划一般项目(2006Z1-E0141)；北京市科技计划一般项目(D07050201170701) |
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| 摘 要： | The role of dinB gene in the appearing of antibiotics resistance was studied. Plasmid containing multi-copy dinB gene was transfected into E. coli to create an overexpression. The strains carrying multi-copies of dinB gene demonstrate a significant survival advantage over the wild strain. In vitro experiment, the dinB-overexpressed strain evolved resistance within 8 hours, while wild strain could not.In vivo experiment with mice model infected with dinB-overexpressed strain, resistant clones emerged significantly earlier and demonstrated significant higher level of resistance than those infected with the wild control strain. The results showed that dinB gene made a contribution in the appearing of the antibiotics resistance and has a potential as a target for prevention from the appearing of antibiotic resistance.
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| 关 键 词： | dinB gene Escherichia coli overexpression |
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