Effects of nitrous oxide on mitochondrial and cell respiration and growth in Distichlis spicata suspension cultures |
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Authors: | Sharon Sowa Leigh E Towill |
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Institution: | (1) USDA-ARS, National Seed Storage Laboratory, Colorado State University, 80523 Fort Collins, CO |
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Abstract: | The reversible inhibition of respiratory activity could provide a novel approach to the preservation of traditionally hard to store plant germplasm such as clonal materials and recalcitrant seed. The gaseous anesthetic nitrous oxide caused a reversible, dose-dependent, partial inhibition of dioxygen utilization in mitochondrial particles isolated from cell suspension cultures of the salt-tolerant marsh grass Distichlis spicata, with maximal inhibition of 33% after 30 minutes exposure to an atmosphere of 80% nitrous oxide plus 20% oxygen. Respiration of whole cells required longer time to be affected by the anesthetic, and was reversibly inhibited an average of 19% when measured using a differential respirometer. Exposure to 80% nitrous oxide plus 20% oxygen for up to 10 days caused no measurable effect on cell growth.Abbreviations PCV
packed cell volume
- EDTA
sodium ethylenediaminetetraacetic acid
- BSA
bovine serum albumin
- MOPS
3(N-morpholino) propanesulfonic acid
- TMPD
N,N,N',N'-tetramethyl-p-phenylene diamine
- STP
standard temperature and pressure |
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Keywords: | anesthetic dioxygen utilization |
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