葎草雄性连锁的RAPD标记的克隆与SCAR标记的建立

应用随机扩增的DNA多态性（RAPD）技术可以快速简便获得雌雄异株植物雌雄株间的基因组差异信息，本文采用该技术对律草雌雄混合基因池进行了扩增，在选用的100条10bD的随机引物中，引物S1519和S2142分别扩增出长度为1207bp和762bp的雄性连锁标记，对其进行回收克隆和测序后发现这两个片段富含AT序列，AT含量分别为64％、54．7％，经核苷酸数据库BLAST检索未发现其同源序列。根据测序结果设计的SCAR引物可以将这两个雄性连锁的RAPD标记转化为稳定性和特异性更好的SCAR标记。

CLONE AND DEVELOPMENT OF RAPD AND SCAR MARKERS LINKED TO SEX DETERMINATION IN THE DIOECIOUS SPECIES Humulus scandens L.

In dioecious plants, the important differences of male and female individuals exist in the genome, so it is very significant to do a comparative study on the sex differences with the DNA markers. In this article, Random Amplification Polymorphic DNA （RAPD） technology was used to increase the Humulus scandens female and male genome teams. The results show that two of the 100 primers, S1519 and S2142, respectly produced a sex-linked band only found in tested males, with the length of 1 207bp and 762bp. These fragments were cloned and sequenced and then aligned with the GenBank database by BLAST, finding that they are abundant in AT （individually 64%, 54.7%）, and no sequence was significantly similar. The RAPD markers were then converted into male-linked SCAR （Sequence Characterized Amplified Regions） markers.