| Institution: | (1) Biosearch Technologies, Inc., 81 Digital Drive, Novato, CA 94949, USA;(2) Berlex Biosciences, 15049 San Pablo Avenue, P.O. Box 4099, Richmond, CA 94804, USA;(3) Berlex Biosciences, 15049 San Pablo Avenue, P.O. Box 4099, Richmond, CA 94804, USA;(4) Present address: Inhale Therapeutic Systems, 150 Industrial Rd., San Carlos, CA, 94070 |
| Abstract: | We describe, and compare, two methods which enable theassembly of proteins from peptide thioester fragmentsprepared by Fmoc chemistry mediated solid phase synthesis. The first, which utilizes iso-thiouronium salts,allows formation of thiophenyl esters directly frompartially protected peptides, either in solution or onresin. The second uses  sulfonamide based safety-catchresins. Data on yields, generality and potential for side-reactions are provided. |
