Genus-specific and phase-dependent effects of nitrate on a sulfate-reducing bacterial community as revealed by dsrB-based DGGE analyses of wastewater reactors |
| |
Authors: | Kouhei Mizuno Yui Morishita Akiko Ando Naofumi Tsuchiya Mai Hirata Kenji Tanaka |
| |
Institution: | (1) Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology, 5-20-1 Shii, Kokuraminami-ku, Kitakyushu 802-0985, Japan;(2) Practical Biotechnology Group, Bioresources Research Center, Research Laboratory, Kyushu Electric Power Co., Inc., 1-10-1, Takagisehigashi, Saga-shi Saga, 849-0922, Japan;(3) Enviroment Department, Kyuden Sangyo Co., Inc., 2-18-20, Najima, Higashi-ku, Fukuoka-shi Fukuoka, 813-0043, Japan;(4) Department of Biological and Environmental Chemistry, School of Humanity-Oriented Science and Engineering, Kinki University, Kayanomori, Iizuka-shi Fukuoka, 820-8555, Japan |
| |
Abstract: | The biogenic production of hydrogen sulfide is a serious problem associated with wastewater treatment. The aim of this study
was to investigate the inhibitory effect of nitrate on the dynamics of sulfate-reducing bacteria (SRB) community in a laboratory-scale
wastewater reactor, originating from a denitrifying plant using activated sludge. For this purpose, denaturing gradient gel
electrophoresis (DGGE) analysis targeting the dsrB (dissimilatory sulfite reductase) gene was used in combination with chemical analyses and measurement of oxidation and reduction
potential (ORP). The reactors were initially dosed with 1.0 and 4.0 g/L potassium nitrate and anaerobically incubated for
490 h. Addition of 4.0 g/L nitrate to the reactor was associated with a prolonged inhibition (over 300 h, i.e., 12.5 days)
of sulfate reduction and this was consistent with a rapid decrease in ORP associated with nitrate depletion. The DGGE analysis
revealed that nitrate addition remarkably attenuated a distinct group of dsrB related to Desulfovibrio, whereas other dsrB groups were not influenced. Furthermore, another sulfate reduction by Syntrophobacter in the later stages of the incubation period occurred in both reactors (regardless of the nitrate concentration), suggesting
that different SRB groups are associated with sulfate reduction at different stages of the wastewater treatment process. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|