Variation among and within Capsicum species revealed by RAPD markers |
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Authors: | J M Rodriguez T Berke L Engle J Nienhuis |
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Institution: | (1) Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA, US;(2) Asian Vegetable Research and Development Center, Shanhua, Tainan, Taiwan, TW |
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Abstract: | Germplasm characterization is an important link between the conservation and utilization of plant genetic resources. A total
of 134 accessions from six Capsicumspecies maintained at the Asian Vegetable Research and Development Center were characterized using 110 randomly amplified
polymorphic DNA (RAPD) markers. Ten pairs of potentially duplicated accessions were identified. Multidimensional scaling analysis
of the genetic distances among accessions resulted in clustering corresponding to a previous species assignment except for
six accessions. Diagnostic RAPDs were identified which discriminate among the Capsicumspecies. The diagnostic markers were employed for improved taxonomic identification of accessions since many morphological
traits used in the identification of Capsicumare difficult to score. Three Capsicumaccessions, misclassified based on morphological traits, were reassigned species status based on diagnostic RAPDs. Three accessions,
not previously classified, were assigned to a species based on diagnostic RAPDs. Definitive conclusions about the species
assignment of three other accessions were not possible. The level of diversity between Capsicum annuumaccessions from the genebank and the breeding program were compared and no differences were observed either for RAPD variation
or diversity. The utilization of genetic resources as a source of variance for useful traits in the breeding program may be
the reason for the similarity of these two groups.
Received: 1 September 1998 / Accepted: 28 December 1998 |
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Keywords: | Capsicum Diagnostic markers Genetic diversity Germplasm RAPD |
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