Molecular typing and antimicrobial resistance of <Emphasis Type="Italic">Salmonella</Emphasis> Enteritidis isolated from poultry,food, and humans in Serbia |
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Authors: | G Kozoderović M Velhner Z Jelesić I Stojanov T Petrović D Stojanović N Golić |
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Institution: | (1) Institute of Public Health of Vojvodina, 21000 Novi Sad, Serbia;(2) Scientific Veterinary Institute “Novi Sad”, 21000 Novi Sad, Serbia;(3) Institute of Molecular Genetics and Genetic Engineering, 11010 Belgrade, Serbia; |
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Abstract: | Molecular typing and resistotyping coupled with gyrA single nucleotide polymorphism (SNP) of 60 Salmonella Enteritidis (SE) isolates originated from poultry, food, and humans in Serbia is described. Molecular fingerprinting was
performed by randomly amplified polymorphic DNA (RAPD) using four primers, and the diversity index (D) was 0.688. In combination with resistotyping and gyrA SNP, D increased to 0.828. A total of 23 genetic groups were obtained. When four RAPD primers were combined, epidemic isolates from
a fast-food restaurant outbreak were clustered in a distinctive genetic group. Among 60 SE strains, three had multiple resistances
to three or more antibiotics. Nine strains were resistant to nalidixic acid (NAL; a non-fluorinated quinolone). The mutations
in quinolone resistance-determining region (QRDR) found in NAL-resistant strains were attributed to Asp87 → Asn in six strains, Asp87 → Gly in one strain, and Ser83 → Phe in one strain. One NAL-resistant strain had no mutations in QRDR, suggesting another mechanism of resistance. |
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