Improvement of pectinase production by interspecific hybrids of Aspergillus strains |
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| Authors: | S Solís & M E Flores C Huitrón |
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| Institution: | Department of Biotechnology, Institute of Biomedical Research, National University of Mexico, UNAM, Mexico |
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| Abstract: | Protoplast fusion induced by polyethylene glycol and Ca2+, was performed between auxotrophic mutants of pectinolytic fungi Aspergillus sp. CH-Y-1043 (A13) ade − and Aspergillus flavipes ATCC-16795 (F7) lys −. Prototrophic colonies were developed on minimal medium with a fusion frequency of 1·0×10−2. The reversion frequency of the mutation in spores and protoplasts was low and ranged from 2·0 to 4·0×10−7. Four prototrophic hybrids (HH, HE, HF and HJ) exhibited enhanced production of endo-pectinase and pectin-lyase. The highest production was observed in HJ ; maximum activities were 150 and 160% respectively, whereas the exo-pectinase production was similar to the wild-type strain Aspergillus sp. CH-Y-1043. Hybrid HJ showed the greatest growth ; nevertheless, specific endo-pectinase and pectin-lyase activities were higher in all hybrids than those produced by the wild-type strains. |
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