Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives |
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Authors: | J Grinsted P M Bennett S Higginson and M H Richmond |
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Institution: | (1) Department of Bacteriology, University of Bristol, University Walk, BS8 1TD Bristol, England |
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Abstract: | Summary The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. Hot spots for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a hot spot, since a particular region does contain many insertions when present in one plasmid but does not do so when part of another. |
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