| 旋毛虫基因重组抗原的纯化 |
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| 引用本文： | 闫玉河,陈辉,许威光,马增全,李春华.旋毛虫基因重组抗原的纯化[J].生物技术,1995(4). |
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| 作者姓名： | 闫玉河 陈辉 许威光 马增全 李春华 |
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| 作者单位： | 河南省农科院畜牧兽医研究所，兽医生物技术国家重点实验室 |
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| 摘 要： | 根据旋毛虫基因重组抗原蛋白的特点，筛选出裂解处理工程菌菌体的方法，并探索了用ＳｅｐｈａｃｒｙｌＳ－３００（ＨＲ）柱层析纯化重组蛋白的程序。用本法处理和纯化后，重组蛋白的纯度可达９０％以上。所纯化的三种重组抗原蛋白均能与猪旋毛虫病血清发生特异性反应而不与正常猪血清和猪囊虫病血清反应，其中以重组蛋白ＲＰ３４的特异性最强，ＲＰ３７较弱，ＲＰ４６介于两者之间。研究结果表明，本纯化方法易于操作、设备简单和特异性蛋白回收率高，是处理和纯化旋毛虫基因重组抗原的最佳程序。
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| 关 键 词： | 旋毛虫 重组抗原 纯化 |
Purification of Recombinant Antigenic Proteins of Trichinella spiralis |
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| Yan Yuhe, Chen Hui, Xu Weiguang,Ma Zengquan, Li Chunhua.Purification of Recombinant Antigenic Proteins of Trichinella spiralis[J].Biotechnology,1995(4). |
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| Authors: | Yan Yuhe Chen Hui Xu Weiguang Ma Zengquan Li Chunhua |
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| Abstract: | The technique of treating host bacteria and procedure for chromatography purification of recombinant proteins using column Sephacryl S-300(HR) were studied on the basis of characterization of T.spiralis recombinant antigenic proteins.Treated and purified with the procedure,the purity of the recombinant proteins could reach as hihg as above 90%.Three kinds of purified antigenic proteins could specifically react with swine antisera against T.spiralis,not with normal swine sera and swine antisera against cysticerci.The recombinant protein RP34 showed the strongest specificity,RP37 was the weakest and RP46 between them.It is indicated that the technique and procedure are easy to perform,need not sophisticated equipment and have a high recovery rate of specific protein,and they are the best way for purification of T.spiralis recombinant antigen. |
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| Keywords: | T spiralis Recombinant antigen Purification |
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