Solution structure and characterization of the DNA-binding activity of the B3BP-Smr domain |
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Authors: | Diercks Tammo AB Eiso Daniels Mark A de Jong Rob N Besseling Rogier Kaptein Robert Folkers Gert E |
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Affiliation: | Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Faculty of Chemistry, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands |
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Abstract: | The MutS1 protein recognizes unpaired bases and initiates mismatch repair, which are essential for high-fidelity DNA replication. The homologous MutS2 protein does not contribute to mismatch repair, but suppresses homologous recombination. MutS2 lacks the damage-recognition domain of MutS1, but contains an additional C-terminal extension: the small MutS-related (Smr) domain. This domain, which is present in both prokaryotes and eukaryotes, has previously been reported to bind to DNA and to possess nicking endonuclease activity. We determine here the solution structure of the functionally active Smr domain of the Bcl3-binding protein (also known as Nedd4-binding protein 2), a protein with unknown function that lacks other domains present in MutS proteins. The Smr domain adopts a two-layer α-β sandwich fold, which has a structural similarity to the C-terminal domain of IF3, the R3H domain, and the N-terminal domain of DNase I. The most conserved residues are located in three loops that form a contiguous, exposed, and positively charged surface with distinct sequence identity for prokaryotic and eukaryotic Smr domains. NMR titration experiments and DNA binding studies using Bcl3-binding protein-Smr domain mutants suggested that these most conserved loop regions participate in DNA binding to single-stranded/double-stranded DNA junctions. Based on the observed DNA-binding-induced multimerization, the structural similarity with both subdomains of DNase I, and the experimentally identified DNA-binding surface, we propose a model for DNA recognition by the Smr domain. |
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Keywords: | Smr, small MutS-related dsDNA, double-stranded DNA ssDNA, single-stranded DNA B3BP, Bcl3-binding protein N4BP2, Nedd4-binding protein 2 EMSA, electrophoretic mobility shift assay GST, glutathione S-transferase EDTA, ethylenediaminetetraacetic acid HSQC, heteronuclear single quantum coherence NOE, nuclear Overhauser enhancement |
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