RFLP mapping of QTLs conferring salt tolerance during the vegetative stage in tomato |
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| Authors: | M R Foolad F Q Chen |
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| Institution: | (1) Department of Horticulture, The Pennsylvania State University, 103 Tyson Building, University Park, PA 16802, USA Fax: +1 814 863-6139 E-mail: mrf5@psu.edu, US |
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| Abstract: | Quantitative trait loci (QTLs) contributing to salt tolerance during the vegetative stage in tomato were investigated using
an interspecific backcross between a salt-sensitive Lycopersicon esculentum breeding line (NC84173, maternal and recurrent parent) and a salt-tolerant Lycopersicon pimpinellifolium accession (LA722). One hundred and nineteen BC1 individuals were genotyped for 151 RFLP markers and a linkage map was constructed. The parental lines and 119 BC1S1 families (self-pollinated progeny of the BC1 individuals) were evaluated for salt tolerance in aerated saline-solution cultures with the salt concentration gradually
raised to 700 mM NaCl+70 mM CaCl2 (equivalent to an electrical conductivity of approximately 64 dS/m and a water potential of approximately −35.2 bars). The
two parental lines were distinctly different in salt tolerance: 80% of the LA722 plants versus 25% of the NC84173 plants survived
for at least 2 weeks after the final salt concentration was reached. The BC1S1 population exhibited a continuous variation, typical of quantitative traits, with the survival rate of the BC1S1 families ranging from 9% to 94% with a mean of 51%. Two QTL mapping techniques, interval mapping (using MAPMAKER/QTL) and single-marker analysis (using QGENE), were used to identify QTLs. The results of both methods were similar and five QTLs were identified on chromosomes 1 (two
QTLs), 3, 5 and 9. Each QTL accounted for between 5.7% and 17.7%, with the combined effects (of all five QTLs) exceeding 46%,
of the total phenotypic variation. All QTLs had the positive QTL alleles from the salt-tolerant parent. Across QTLs, the effects
were mainly additive in nature. Digenic epistatic interactions were evident among several QTL-linked and QTL-unlinked markers.
The overall results indicate that tomato salt tolerance during the vegetative stage could be improved by marker-assisted selection
using interspecific variation.
Received: 4 January 1999 / Accepted: 4 January 1999 |
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| Keywords: | Lycopersicon esculentum L pimpinellifolium Salt tolerance Vegetative growth Restriction fragment length polymorphism (RFLP) Quantitative trait loci (QTLs) |
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