An attempt to design an isoluminol-hydrogen peroxidase-amplified CL that measures intracellularly produced H2O2 in phagocytes: sensitivity for H2O2 is not high enough to allow detection. |
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| Authors: | Anna-Lena Stenfeldt Claes Dahlgren |
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| Affiliation: | 1. Department of Rheumatology and Inflammation Research, University of G?teborg, Sweden;2. Department of Clinical Bacteriology, University of G?teborg, Sweden |
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| Abstract: | A technique was designed for the determination of hydrogen peroxide release. We found, however, that the isoluminol-amplified chemiluminescence technique is a suitable tool for measuring secretion of superoxide but not hydrogen peroxide. |
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| Keywords: | chemiluminescence hydrogen peroxide production neutrophils superoxide production isoluminol |
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