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Study on fibroin heavy chain of the silkwormBombyx mori by fluorescencein situ hybridization (FISH)
Authors:Fangzhou Song  Pingbo Zhang  Faping Yi  Xijun Hong  Cheng Lu  Banno Yutaka  Fujii Hiroshi and Koga Katsumi
Institution:1. College of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016, China;Key Sericultural Laboratory of Agriculture Ministry, Southwest Agricultural University, Chongqing 400716, China
2. Key Sericultural Laboratory of Agriculture Ministry, Southwest Agricultural University, Chongqing 400716, China;School of Life Science, Southwest China Normal University, Chongqing 400715, China
3. School of Life Science, Southwest China Normal University, Chongqing 400715, China
4. Key Sericultural Laboratory of Agriculture Ministry, Southwest Agricultural University, Chongqing 400716, China
5. Faculty of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan
Abstract:The sericulture industry plays a very important role in our national economy. Silkworm (Bombyx mori) is always regarded as a model animal and biological reactor. There have been detailed studies on the structure, expression and control and molecular evolution of silk genes. However, few, if any, reports are available on the localization of structural genes in silkworm by molecular cytogenetics. The present experiment has tentatively localized theFib-H gene at the distal end of the 25th linkage group, namely at the 25-0.0 position, and verified thatFib-H has only one locus, thus providing a temporary solution to the problem about its localization.
Keywords:silkworm Bombyx mori  fibroin gene  fluorescence in situ hybridization  gene localization
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