Cloning and characterization of the rDNA repeat unit of Podospora anserina |
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| Authors: | N Labat M Perrot and J Bégueret |
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| Institution: | (1) Laboratoire de Génétique, allée des Facultés, F-33405 Talence-Cedex, France |
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| Abstract: | Summary DNA coding for ribosomal RNA in Podospora anserina has been cloned and was found as a tandemly repeated 8.3 kb sequence. The cloned rDNA was characterized by restriction endonuclease mapping. The location of 5.8S, 18S and 28S rRNA coding regions was established by DNA-RNA hybridization and S1 nuclease mapping. The organization of P. anserina rRNA genes is similar to that of Neurospora crassa and Aspergillus nidulans. The rDNA unit does not contain the sequence coding for 5S RNA. |
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