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A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaks |
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| Authors: | María Moreno-Villanueva Ragen Pfeiffer Thilo Sindlinger Alan Leake Marcus Müller Thomas BL Kirkwood Alexander Bürkle |
| Institution: | 1. Lehrstuhl Molekulare Toxikologie, Fachbereich Biologie, Universit?t Konstanz, D-78457, Konstanz, Germany 2. Institute for Ageing and Health, Henry Wellcome Laboratory for Biogerontology Research, Newcastle University, Campus for Ageing and Vitality, Newcastle upon Tyne, NE4 5PL, UK 3. Abteilung Tumorvirologie, Deutsches Krebsforschungszentrum, D-69120, Heidelberg, Germany 4. TherapySelect GmbH & Co. KG, Im Neuenheimer Feld 584, D-69120, Heidelberg, Germany |
| Abstract: | BackgroundFormation and repair of DNA single-strand breaks are important parameters in the assessment of DNA damage and repair occurring in live cells. The 'Fluorimetric Detection of Alkaline DNA Unwinding (FADU)' method Birnboim HC, Jevcak JJ. Cancer Res (1981) 41:1889–1892] is a sensitive procedure to quantify DNA strand breaks, yet it is very tedious to perform. |
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