Application of constrained regularization analysis to low-angle quasi-elastic light scattering

A constrained regularization procedure has been applied to a low-angle quasi-elastic light scattering system in order to determine particle size distributions. The conditions under which this procedure may be successfully applied to low-angle photon correlation spectroscopy have been characterized. Acquisition of photon count data over a short time period, relative to the long exponential decay constants of correlation functions obtained at low forward angles, resulted in particle size distributions which were stable with regard to peak width and weighted mean particle radius. Irrespective of the number of photon counts obtained, peak resolution and position on the particle size scale were not optimized unless anomalies in the correlation function due to transient increases in the mean photon counting rate were removed from the photon count data prior to autocorrelation. When such measures were taken, reasonable size distributions were obtained for well characterized protein standards and for liposomal suspensions.