双孢蘑菇子实体发育后期差异表达蛋白质分析 |
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引用本文: | 陈美元,廖剑华,李洪荣,蔡志欣,郭仲杰,王泽生.双孢蘑菇子实体发育后期差异表达蛋白质分析[J].菌物学报,2013,32(5):855-861. |
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作者姓名: | 陈美元 廖剑华 李洪荣 蔡志欣 郭仲杰 王泽生 |
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作者单位: | 福建省农业科学院食用菌研究所 福建 福州 350014;福建省农业科学院食用菌研究所 福建 福州 350014;福建省农业科学院食用菌研究所 福建 福州 350014;福建省农业科学院食用菌研究所 福建 福州 350014;福建省农业科学院食用菌研究所 福建 福州 350014;福建省农业科学院食用菌研究所 福建 福州 350014 |
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基金项目: | 福建省公益类科研专项项目(No. 2011R1022-1);福建省自然科学基金(No. 2012J01108) |
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摘 要: | 为探讨双孢蘑菇子实体发育后期的蛋白质表达变化,对双孢蘑菇As2796子实体采收期、成熟期和开伞期的蛋白质组进行了双向电泳(2-DE)分析,发现了16个表达差异明显的蛋白质。通过质谱分析(MALDI-TOF/TOF MS)和数据库检索,有14个差异蛋白质获得鉴定。其中磷酸烯醇式丙酮酸水合酶与能量代谢相关,T-蛋白复合体1、蛋白酶体、5-甲基四氢三谷氨酸-同型半胱氨酸甲基转移酶、1-吡咯琳-5-羧酸脱氢酶、精氨酸酶与氨基酸或蛋白质代谢直接相关,而GTP结合蛋白则参与细胞的多种生命活动,在细胞的生长发育过程中起着重要的作用。另外7个为功能未知的蛋白质。
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关 键 词: | 食用菌 蛋白质组 双向电泳 质谱分析 蛋白质鉴定 |
Analysis of differentially expressed proteins in later developing stage fruitbody of Agaricus bisporus |
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Authors: | CHEN Mei-Yuan LIAO Jian-Hu LI Hong-Rong CAI Zhi-Xin GUO Zhong-Jie and WANG Ze-Sheng |
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Institution: | Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China;Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China;Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China;Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China;Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China;Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350014, China |
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Abstract: | To discuss the protein expression changes in later developing fruitbody of Agaricus bisporus, the technique of two-dimensional electrophoresis (2-DE) was used to analyze the protein expression differences among the harvesting, maturity and opening stage of A. bisporus As2796 fruitbody. As a result, sixteen proteins were found to be differentially expressed. Based on the results of MALDI-TOF/TOF MS analysis and database searching, fourteen proteins were identified successfully. Among them phosphopyruvate hydratase is related to energy metabolism, and T-complex protein 1, proteasome, 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, 1-pyrroline-5-carboxylate dehydrogenase, arginase are all directly related to amino acid or protein metabolism, while GTP-binding protein is related to several intracellular bioprocess, and play important roles in cellular growth, development, differentiation etc. Seven others were unknown proteins. |
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Keywords: | edible fungus proteome two-dimensional electrophoresis (2-DE) MALDI-TOF/TOF mass spectrometry (MS) protein identification |
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