| 用Real-Time PCR评价花生田间金龟子绿僵菌的存活能力 |
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| 引用本文： | 李兴佳,农向群,曹广春,刘迅,王广君,张泽华.用Real-Time PCR评价花生田间金龟子绿僵菌的存活能力[J].菌物学报,2013,32(4):710-720. |
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| 作者姓名： | 李兴佳 农向群 曹广春 刘迅 王广君 张泽华 |
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| 作者单位： | 中国农业科学院植物保护研究所 农业部作物有害生物综合治理重点实验室 北京 100081 |
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| 基金项目： | 公益性（农业）行业专项（No. 201003025）；农业部948计划（No. 2011-G4）；现代农业产业体系（No. CARS-35） |
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| 摘 要： | 为了能够对金龟子绿僵菌在花生田间的存活能力进行精确的定量研究,通过比对真菌ITS序列,设计出针对金龟子绿僵菌的特异性引物,并建立金龟子绿僵菌Real-Time PCR反应体系。结果表明,标准品在拷贝数为8.49×103–8.49×109copies/μL之间,线性关系良好,线性方程为y=-3.257x+6.969,相关系数为R=0.9980,扩增效率E=102.8%,检出痕量为20个孢子/g土壤。以Real-Time PCR方法和平板稀释法,分别对花生根部施用金龟子绿僵菌后不同时期的土壤进行定量分析,结果表明两种方法检测的金龟子绿僵菌数量变化趋势相似,施用的金龟子绿僵菌在根围与根际都呈现先快速下降后缓慢回升的趋势,90d时金龟子绿僵菌DNA量和CFU值均下降至初始的10%以下,之后出现回升;根际的金龟子绿僵菌相对地下降较慢而回升较快,在120d时可恢复到初始的52.17%和38.65%,显著高于根围的数量。试验建立的Real-Time PCR体系可用于金龟子绿僵菌土壤宿存的定量检测,而且适用于低含量的检测。
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| 关 键 词： | 金龟子绿僵菌 种群动态 定量分析 |
Evaluating survival ability of Metarhizium anisopliae in the peanut field by Real-Time PCR |
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| Authors: | LI Xing-Ji NONG Xiang-Qun CAO Guang-Chun LIU Xun WANG Guang-Jun and ZHANG Ze-Hua |
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| Institution: | Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agrucultural Science, Beijing 100081, China;Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agrucultural Science, Beijing 100081, China;Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agrucultural Science, Beijing 100081, China;Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agrucultural Science, Beijing 100081, China;Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agrucultural Science, Beijing 100081, China;Key Laboratory of Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agrucultural Science, Beijing 100081, China |
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| Abstract: | |
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| Keywords: | Metarhizium anisopliae population dynamic quantitative analysis |
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