| 银杏内生真菌多样性研究(英文) |
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| 引用本文： | 郑建华,康冀川,雷帮星,李启瑞,文庭池,孟泽彬.银杏内生真菌多样性研究(英文)[J].菌物学报,2013,32(4):671-681. |
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| 作者姓名： | 郑建华 康冀川 雷帮星 李启瑞 文庭池 孟泽彬 |
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| 作者单位： | 1. 西南药用生物资源教育部工程研究中心 贵州贵阳 550025;贵州大学贵州省生化工程中心 贵州贵阳 550025;贵州大学生命科学学院 贵州贵阳 550025
2. 西南药用生物资源教育部工程研究中心 贵州贵阳 550025;贵州大学贵州省生化工程中心 贵州贵阳 550025 |
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| 基金项目： | grant from the Science and Technology Plan Projects of Guizhou Province (No. [2010] 4009); the Foundation of Science and Technology Bureau of Guiyang City, China (No. [2002] 2157); the International Plan of Science and Technology, Guizhou Province (No. [2012] 7006). |
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| 摘 要： | 采用组织块分离法,从中国福建、江苏、贵州三省银杏Ginkgo biloba的根、茎、叶、树皮组织中分离内生真菌,利用形态学与ITS rDNA序列分析相结合的方法对所分离的菌株进行鉴定。结果表明从根、茎、叶和树皮分离出175株内生真菌,归为47类,每一类取代表菌株进行ITS测序及系统分析,分别属于子囊菌门的8个目,即Eurotiales、Hypocreales、Xylariales、Trichosphaeriales、Glomerellales、Diaporthales、Botryosphaeriales、Pleosporales,11科,16属。其中刺盘孢属Colletotrichum(19.75%)、链格孢属Alternaria(19.15%)、镰孢菌属Fusarium(10.64%)和拟茎点霉属Phomopsis(10.64%)为优势菌群;并且新丛赤壳属Neonectria和生赤壳属Bionectria为首次从银杏中分离出。Shannon-Wiener指数(H=2.4192)和Simpson指数(1-D=0.8856)的计算结果反应出所获得的银杏内生真菌菌群具有较高的多样性。
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| 关 键 词： | 银杏 内生真菌 多样性指数 ITS rDNA序列 |
Diversity of endophytic fungi associated with Ginkgo biloba |
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| Authors: | ZHENG Jian-Hu KANG Ji-Chuan LEI Bang-Xing LI Qi-Rui WEN Ting-Chi and MENG Ze-Bin |
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| Institution: | The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University, Guiyang, Guizhou 550025, China
The Research Center of Biochem-engineering at Guizhou, Guizhou University, Guiyang, Guizhou 550025, China
College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China;The Research Center of Biochem-engineering at Guizhou, Guizhou University, Guiyang, Guizhou 550025, China
College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China;The Research Center of Biochem-engineering at Guizhou, Guizhou University, Guiyang, Guizhou 550025, China
College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China;The Research Center of Biochem-engineering at Guizhou, Guizhou University, Guiyang, Guizhou 550025, China
College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China;The Research Center of Biochem-engineering at Guizhou, Guizhou University, Guiyang, Guizhou 550025, China
College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China;The Research Center of Biochem-engineering at Guizhou, Guizhou University, Guiyang, Guizhou 550025, China
College of Life Sciences, Guizhou University, Guiyang, Guizhou 550025, China |
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| Abstract: | The endophytic fungi were isolated from samples of roots, branches, leaves and bark of Ginkgo biloba from Fujian, Jiangsu and Guizhou provinces in China. They were identified based on morphology and ITS rDNA sequences. Amongst the 175 isolates, 47 groups were erected with their morphological characteristics. One representative isolate of each group was selected for ITS sequencing and phylogenetic analysis. The results showed that all the fungal isolates belonged to eight different fungal groups, namely Eurotiales, Hypocreales, Xylariales, Trichosphaeriales, Glomerellales, Diaporthales, Botryosphaeriales and Pleosporales in Ascomycota. Colletotrichum, Alternaria, Fusarium and Phomopsis were the dominant genera. For the first time, the genera Neonectria and Bionectria were isolated from G. biloba. The Shannon-Wiener and Simpson diversity index values for the overall fungal community were H=2.4192 and 1-D=0.8856, respectively, which suggest that G. biloba harbors highly diverse mycota. |
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| Keywords: | Ginkgo biloba endophytic fungi diversity index ITS rDNA sequence |
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