Archaebacterial elongation factor Tu insensitive to pulvomycin and kirromycin |
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| Authors: | P Cammarano A Teichner G Chinali P Londei M de Rosa A Gambacorta B Nicolaus |
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| Institution: | Laboratories for the Studies of Metabolic Disorders, Howard Hughes Medical Institute and Department of Physiology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA |
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| Abstract: | A spermine-dependent, polyphenylalanine-synthesizing cell-free system having an optimum activity at 75-85 degrees C, has been developed from the extremely thermoacidophilic archaebacterium Caldariella acidophila. The C. acidophila system is totally insensitive to the EF-Tu targeted antibiotics pulvomycin (at 40 degrees C) and kirromycin (at 47-72 degrees C) contrary to control systems derived from both mesophilic (Escherichia coli) and thermoacidophilic (Bacillus acidocaldarius) eubacteria. The archaebacterial EF-Tu-equivalent factor is also immunologically unrelated to eubacterial EF-Tu and does not cross react with antibodies against Escherichia coli EF-Tu. The pulvomycin and kirromycin reactions thus provide new phyletic markers for archaebacterial ancestry. |
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| Keywords: | Archaebacteria Elongation factor Tu Pulvomycin Kirromycin DTT dithiothreitol aa-tRNA aminoacyl-tRNA EF elongation factor |
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