Determination of the pheromone-producing region that has epoxidation activity in the abdominal tip of the Japanese giant looper, Ascotis selenaria cretacea (Lepidoptera: Geometridae) |
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Authors: | Fujii Takeshi Suzuki Masataka G Kawai Takeshi Tsuneizumi Kazuhide Ohnishi Atsushi Kurihara Masaaki Matsumoto Shogo Ando Tetsu |
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Affiliation: | Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo, University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan. |
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Abstract: | The epoxydienyl sex pheromone of Ascotis selenaria cretacea can be detected only within a rod-like abdominal tip (RAT) of the female. To clarify which part of the RAT is the sex pheromone-producing region, the RAT was morphologically divided into three sections, defined positionally from the abdomen as sections A, B, and C. GC-MS measurements clearly showed that the sex pheromone compound levels in section B were four times greater than those of the other sections. Microscopic dissection analysis revealed that section B consists of four tissues: rectum, oviduct, musculature, and intersegmental membrane. GC-MS analysis of the individual tissues revealed that approximately 90% of the sex pheromone in section B is localized in the intersegmental membrane. A cell layer was found in the intersegmental membrane after staining with propidium iodide. Furthermore, incubation of tissues dissected from section B with a deuterated trienyl pheromone precursor revealed that the labeled epoxy pheromonal component was detected exclusively in the intersegmental membrane. We have determined that the sex pheromone-producing region of A. s. cretacea is on the terminal side of the intersegmental membrane located between the 8th and 9th abdominal segments. |
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Keywords: | Pheromone gland Abdominal tip morphology Sex pheromone biosynthesis Triene epoxidation Geometrid moth |
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