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| L－异亮氨酸产生菌选育的研究 | |
| 引用本文： | 张伟国. L－异亮氨酸产生菌选育的研究[J]. 氨基酸和生物资源, 1996, 18(3): 1-5 |
| 作者姓名： | 张伟国 |
| 作者单位： | ;1.无锡轻工大学 |
| 摘 要： | 以黄色短杆菌（Ｂｒｅｖｉｂａｃｔｅｒｉｕｍｆｌａｖｕｍ）ＡＴＣＣ１４０６７为出发菌株，经硫酸二乙酯（ＤＥＳ）、紫外线（ＵＶ）和亚硝基胍（ＮＴＧ）逐级诱变处理，α－氨基－β－羟基戊酸（ＡＨＶ）、Ｓ－２－氨基乙基－Ｌ－半胱氨酸（ＡＥＣ）、磺胺胍（ＳＧ）、乙硫氨酸（Ｅｔｈ）、α－氨基丁酸（α－ＡＢ）、异亮氨酸氧肟酸（ＩｌｅＨｘ）等氨基酸结构类似物及琥珀酸为碳源平板定向筛选，获得一株Ｌ－异亮氨酸高产菌ＺＱ－４（ＡＨＶ~γ、ＡＥＣ~γ、ＳＡＭ~γ、ＳＧ~γ、Ｅｔｈ~γ、α－ＡＢ~γ、ＩｌｅＨｘ~γ）在含１３．５％葡萄糖培养基中，摇瓶发酵７２ｈ、Ｌ－异亮氨酸积累可达２．８－３．０％。 |
| 关 键 词： | L－异亮氨酸，育种，黄色短杆菌 |
Breeding of L-Isoleucine Producing Mutant of Brevibacterium flavum |
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| Affiliation: | ,Zhang Weiguo(Wuxi University of Light Industry Wuxi 214036) |
| Abstract: | Brevibacterium flavum ATCC14067 as a starting strain was stepwise treated with diethylsufate(DES), ultraviolet (UV) and N-methyl-N'-nitrosonguanidine (NTG). A L-isoleucine over-producing mutant,ZQ-4,was obtained, which could resist to α-amino-β-hydroxyvalic acid (AHV),S-(β-aminoethyl)-L-cysteine (AEC), sulfaguanidine (SG), ethionine (Eth), α-amino-butyric acid (α-AB),isoleucine-hydroxamate (IleHx) and could grow quickly on succinate (as sole carbon source) medium. ZQ-4 could accumulate 2.8-3.0% of L-isoleucine in a medium containing 13.5% glucose shaking at 31±1℃ for 72hrs'. |
| Keywords: | L-Isoleucine Breeding Brevibacterium flavum |
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