Hydrolytic enzyme activities in germinating spores ofAdiantum capillus-veneris L. |
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Authors: | Tomokazu Koshiba Takao Minamikawa Masamitsu Wada |
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Institution: | (1) Department of Biology, Tokyo Metropolitan University, Fukazawa 2-1, Setagaya-ku, 158 Tokyo |
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Abstract: | Changes in hydrolytic enzyme activities were investigated during spore germination ofAdiantum capillus-veneris L. The spores were incubated for 3 days in the dark at 25 C for imbibition, and then germination of the spores was induced
by continuous irradiation with red light. At day 2 after onset of the red light irradiation, rhizoids appeared out of spore
coats and protonemal cells became visible on the following day. Lipase occurred in dry spores and its activity decreased during
3 days of dark incubation. The activity started to increase when the spore germination was induced by red light irradiation.
On the other hand, amylolytic and aminopeptidase activities which were also detected in dry spores decreased continuously
during the dark incubation and following the germination process. RNase activity also decreased during 3 days of dark incubation
but the activity was retained thereafter at a constant level with or without red light irradiation. Developmental patterns
of these hydrolytic enzymes were classified into two groups: One decreased during imbibition and dark incubation but increased
after red light irradiation and the other continuously decreased during dark incubation and germination. These results are
discussed in relation to compositional changes of cell constitutions such as lipid, sugars, proteins and amino acids during
spore germination. |
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Keywords: | Adiantum capillus-veneris L (fern) Aminopeptidase Amylase Spore germination (fern) Hydrolytic enzymes Lipase |
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