| 敲低前列腺癌相关基因PC-1的表达抑制前列腺癌细胞C4-2雄激素非依赖性生长 |
| |
| 引用本文： | 俞岚,钱晓龙,施庆国,李山虎,王洪涛,王乐乐,周建光.敲低前列腺癌相关基因PC-1的表达抑制前列腺癌细胞C4-2雄激素非依赖性生长[J].生物技术通讯,2010,21(4):478-480. |
| |
| 作者姓名： | 俞岚 钱晓龙 施庆国 李山虎 王洪涛 王乐乐 周建光 |
| |
| 作者单位： | 军事医学科学院,生物工程研究所,北京,100850 |
| |
| 基金项目： | 国家自然科学基金，国家高技术研究发展计划 |
| |
| 摘 要： | 目的:研究敲低癌基因D52家族成员PC-1的表达对前列腺癌细胞雄激素非依赖性生长的影响。方法:利用RNA干扰技术构建PC-1稳定低表达的C4-2细胞株;利用四唑盐(MTT)比色实验检测敲低PC-1基因表达对C4-2细胞雄激素非依赖生长的影响。结果:敲低PC-1表达抑制前列腺癌C4-2细胞的生长,并降低了C4-2细胞雄激素非依赖性生长的能力。结论:PC-1基因参与了前列腺癌向雄激素非依赖阶段发展和维持的过程,为进一步研究PC-1在促进前列腺癌细胞发生发展过程中的作用奠定了基础。
|
| 关 键 词： | 前列腺癌 前列腺癌相关基因PC-1 RNA干扰 C4-2细胞 雄激素非依赖性生长 |
Silencing of PC-1 Inhibits the Androgen-Independent Growth of Prostate Cancer Cell Line C4-2 |
| |
| YU Lan,QIAN Xiao-Long,SHI Qing-Guo,LI Shan-Hu,WANG Hong-Tao,WANG Le-Le,ZHOU Jian-Guang.Silencing of PC-1 Inhibits the Androgen-Independent Growth of Prostate Cancer Cell Line C4-2[J].Letters in Biotechnology,2010,21(4):478-480. |
| |
| Authors: | YU Lan QIAN Xiao-Long SHI Qing-Guo LI Shan-Hu WANG Hong-Tao WANG Le-Le ZHOU Jian-Guang |
| |
| Institution: | YU Lan,QIAN Xiao-Long,SHI Qing-Guo,LI Shan-Hu,WANG Hong-Tao,WANG Le-Le,ZHOU Jian-Guang Laboratory of Medical Molecular Biology,Beijing Institute of Biotecnology,Beijing 100850,China |
| |
| Abstract: | Objective: PC-1 belongs to tumor protein D52 (TPD52) family. This study was to assess the contribution of silencing of PC-1 to androgen-independent growth of prostate cancer cells. Methods: Prostate cancer cell line C4-2 with stable low expression of PC-1 gene by RNA interference was established. MTT assays were applied to observe the impact of PC-1 gene knockdown on the androgen-independent growth of C4-2 cells. Results: Silencing of PC-1 Inhibits the androgen-independent growth of C4-2 cells. Conclusion: ... |
| |
| Keywords: | prostate cancer prostate and colon gene 1(PC-1) RNA interference C4-2 cell line androgen-independent growth |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
