Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline |
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| Authors: | SI Pitta-Alvarez TC Spollansky AM Giulietti |
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| Institution: | (1) Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956 6to piso, (1113) Buenos Aires, Argentina |
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| Abstract: | CaCl2 (50 mM) and hemicellulase (0.5 U mg–1) increased the intracellular accumulation (60–250%), release (60–200%) and production (45–200%) of hyoscyamine and scopolamine in hairy roots of Brugmansia candida. Theophylline (0.25 mM), alone or in combination with hemicellulase, was ineffective in increasing hyoscyamine and scopolamine production. |
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| Keywords: | Brugmansia candida hairy root cultures hyoscyamine scopolamine |
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