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| 丙二醛对大鼠海马神经元结构的破坏和钙离子稳态的影响 | |
| 引用本文： | 蔡建光,汤华,唐晖,印大中. 丙二醛对大鼠海马神经元结构的破坏和钙离子稳态的影响[J]. 生命科学研究, 2011, 15(4): 283-289 |
| 作者姓名： | 蔡建光 汤华 唐晖 印大中 |
| 作者单位： | 1. 湖南科技大学体育学院,中国湖南湘潭,410200 2. 湖南城市学院体育系,中国湖南益阳,410300 3. 湖南师范大学生命科学学院,中国湖南长沙,410081 |
| 基金项目： | 国家自然科学基金资助项目(30971413); 湖南科技大学博士启动基金资助项目(E51090) |
| 摘 要： | 脂质过氧化中间产物丙二醛(Malondialdehyde,MDA)在生物体内表现了广泛的生物毒性,MDA也是机体过度训练和运动性疲劳的重要生理指标.采用光学显微镜和透射式电子显微镜观察不同浓度MDA作用后海马神经元形态和超微结构的变化,并采用荧光分光光度法测定原代培养的海马神经元中Ca2+-ATPase活性的变化和胞质游离钙离子水平的变化,探讨MDA对海马神经元形态和结构上的破坏及神经元钙离子稳态的影响.在光镜下可观察到MDA作用下神经元突触变短,胞体肿胀,出现细胞死亡或凋亡的形态特征;在电镜下可观察到线粒体结构的明显破坏,内膜上的嵴颗粒减少或消失;同时MDA还通过抑制质膜Ca2+-ATPase的活性和其它的途径,破坏神经元胞质游离Ca2+稳态.结果表明,MDA可通过破坏海马神经元的结构和影响胞质中钙离子稳态,破坏神经元的生理功能,在机体运动性中枢疲劳形成中可能发挥重要作用. |
| 关 键 词： | 丙二醛 运动性疲劳 海马神经元 钙离子稳态 |
The Effects of Malondialdehyde on the Cellular Structures of Hippocampal Neurons and Its Calcium Homeostasis in SD Rats |
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| CAI Jian-guang,TANG Hua,TANG Hui,YIN Da-zhong. The Effects of Malondialdehyde on the Cellular Structures of Hippocampal Neurons and Its Calcium Homeostasis in SD Rats[J]. Life Science Research, 2011, 15(4): 283-289 | |
| Authors: | CAI Jian-guang TANG Hua TANG Hui YIN Da-zhong |
| Affiliation: | CAI Jian-guang1,TANG Hua2,TANG Hui1,YIN Da-zhong3(1.School of Physical Education,Hunan University of Science & Technology,Xiangtan 410200,Hunan,China,2.Department of Physical Education,Hunan City University,Yiyang 413000,3.College of Life Sciences,Hunan Normal University,Changsha 410081,China) |
| Abstract: | As a useful physiological index for over trained or exercise-induced fatigue,there were kinds of biological toxicity of malondialdehyde(MDA) produced in the process of lipid peroxidation.In this investiga-tion,in order to observe the effects of MDA on the damages of hippocampal neuronal shapes and ultra-struc-ture,and examine the calcium homeostasis in primary culture neurons,the microscope and transmission electronic microscope were applied for observing the changes of shapes and the transforms of ultra-st... |
| Keywords: | malondialdehyde exercise-induced fatigue hippocampal neurons calcium homeostasis |
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