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Production of recombinant hirudin in galactokinase-deficientSaccharomyces cerevisiae by fed-batch fermentation with continuous glucose feeding
Authors:Srinivas Ramisetti  Hyun Ah Kang  Sang Ki Rhee  Chul Ho Kim
Affiliation:(1) Metabolic Engineering Research Laboratory, Korea Research Institute of Bioscience and Biotechnology, 52, Oun-dong, Yusong, 305-333 Daejeon, Korea
Abstract:The artificial gene coding for anticoagulant hirudin was placed under the control of theGAL10 promoter and expressed in the galactokinase-deficient strain (Δgal1) ofSaccharomyces cereivisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.
Keywords:recombinant hirudin   Saccharomyces cerevisiae    GAL promoter  galactose  fed-batch fermentation
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