A simple and rapid method of quantitative analysis of phosphoamino acids by high-performance liquid chromatography |
| |
| Authors: | N Morrice A Aitken |
| |
| Affiliation: | 1. Service de Biochimie, Hôpital A. Trousseau, APHP, 75012 Paris, France;2. Laboratoire de métabolomique clinique Hôpital Saint Antoine, 75012 Paris, France;3. Thermo Fisher Scientific, Courtaboeuf, 91000, France;4. Université Paris-Saclay, Institut de Chimie Physique, CNRS – UMR 8000 and IUT d''Orsay, Plateau de Moulon, 91400 France |
| |
| Abstract: | A high-performance liquid chromatographic system for the separation of nonradiolabeled phosphoamino acids and orthophosphate by ion-pair reverse-phase chromatography has been developed. By the use of low-ionic-strength phthalate buffers at pH 6.3, the phosphoamino acids can be visualized by virtue of this uv-active eluant. The technique is sensitive to 200 pmol of phosphoamino acid and has been shown to be directly applicable to the analysis of isolated phosphoproteins. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
