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Development of a freeze-drying protocol for the long-term storage of S9-fraction at ambient temperatures |
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| Authors: | Sebastian Buchinger Elisabeth Campen Valeri Morosow Georg Reifferscheid |
| Institution: | a Department of Ecotoxicology and Biochemistry, Federal Institute of Hydrology (BfG), Am Mainzer Tor 1, 56068 Koblenz, Germany b University of Applied Sciences Trier, Umweltcampus Birkenfeld, 55761 Birkenfeld, Germany c University of Applied Sciences, Life Sciences and Engineering, Bingen, 55411 Bingen, Germany |
| Abstract: | The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab independent device for the detection of genotoxic compounds in field studies with an integrated module for the metabolic activation of pre-genotoxic compounds.The carbohydrates sucrose, trehalose and raffinose were tested in different concentrations or mixtures in order to increase the product stability of the S9-fraction during and after freeze-drying. The activity of the freeze-dried S9-samples was evaluated by means of their potential to activate pre-genotoxic compounds. The successful long-term stabilization of enzymes of the rodent liver S9-fraction for 6 weeks at room temperature by freeze-drying in the presence of 250 mM trehalose is presented. |
| Keywords: | S9-fraction Long-term stabilization SOS-umu-test Genotoxicity Biosensor |
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