Mechanosensitive Properties of BK Channels from Embryonic Rat Neuroepithelium |
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| Authors: | J-M Mienville JL Barker GD Lange |
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| Institution: | (1) Laboratory of Neurophysiology, LNP, NINDS, NIH, Building 36, Room 2C02 36 Convent Dr MSC 4066 Bethesda MD 20892-4066, US;(2) Instrumentation and Computer Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, US |
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| Abstract: | The mechanosensitive properties of large-conductance Ca2+-activated K+ (BK) channels from embryonic rat neuroepithelium were investigated with the cell-attached and inside-out configurations of
the patch-clamp technique. The channels were activated in both recording configurations by negative pressures applied to the
patch electrode, but reversal of the effect was total and immediate in inside-out patches whereas it was incomplete and delayed
in on-cell patches. This mechanosensitivity was not mediated by Ca2+ ions or fatty acids, suggesting that it is an intrinsic property of these channels. Cytochalasin B did not affect mechanosensitivity
in on-cell patches but increased it in inside-out patches. Kinetic studies showed that stretch increased the mean open time
of the channels and decreased the slowest time constant of their closed-time distributions. The present as well as previous
results suggest complex interactions between embryonic BK channels and their membranous and submembranous environment.
Received: 1 February 1996/Revised: 25 March 1996 |
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| Keywords: | : Ca2+-activated K+ channels — Stretch — Mechanosensitivity — Cytoskeleton — Rat Neuroepithelium — Patch clamp |
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