Callus induction and somatic embryogenesis of Phalaenopsis |
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Authors: | Y Ishii T Takamura M Goi M Tanaka |
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Institution: | (1) Department of Horticulture, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan, JP |
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Abstract: | Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with
sucrose. The optimal concentration of sucrose was 40 g ⋅ l–1. Medium containing 200 ml ⋅ l–1 coconut water together with 40 g ⋅ l–1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The
calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the
PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
Received: 11 June 1997 / Revision received: 6 October 1997 / Accepted: 18 October 1997 |
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Keywords: | Phalaenopsis Protocorm-like bodies Callus induction Somatic embryogenesis |
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