Enhancement ofcis,cis-muconate productivity by overexpression of catechol 1,2-dioxygenase inPseudomonas putida BCM114 |
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| Authors: | Beum Jun Kim Won Jae Choi Eun Yeol Lee Cha Yong Choi |
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| Affiliation: | (1) Department of Chemical Technology, College of Engineering, Seoul National University, 151-742 Seoul, Korea;(2) Department of Food Science and Technology, College of Engineering, Kyungsung University, 608-736 Pusan, Korea |
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| Abstract: | For enhancement ofcis,cis-muconate productivity from benzoate, catechol 1,2-dioxygenase (C12O) which catalyzes the rate-limiting step (catechol conversion tocis,cis-muconate) was cloned and expressed in recombinantPseudomonas putida BCM114. At higher benzoate concentrations (more than 15 mM),cis,cis-muconate productivity gradually decreased and unconverted catechol was accumulated up to 10 mM in the case of wildtypeP. putida BM014, whereascis,cis-muconate productivity continuously increased and catechol was completely transformed tocis,cis-muconate forP. putida BCM114. Specific C12O activity ofP. putida BCM114 was about three times higher than that ofP. putida BM014, and productivity was enhanced more than two times. |
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| Keywords: | Pseudomonas putida BCM114 cis,cis-muconate catA cloning catechol 1,2-dioxygenase |
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