Methods For Removing Uranyl Acetate Precipitate From Ultrathin Sections |
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Authors: | S W Avery E A Ellis |
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Institution: |
a Insects Affecting Man Research Laboratory, Agricultural Research Service, USDA, Gainesville, Florida
b Department of Entomology and Nematology, University of Florida, Gainesville, Florida |
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Abstract: | Precipitate resulting from en bloc staining with uranyl acetate was removed by treating sections with 15% oxalic acid in 50% methanol for 30 minutes at 40 C. Precipitate resulting from poststaining sections with hot uranyl acetate was removed by rinsing sections in 0.25-0.50% aqueous oxalic acid for 10-15 seconds at room temperature. Rinsing sections for longer than 30 seconds removed uranyl precipitate and also destained the sections. These procedures did not damage the embedding medium or cellular detail. |
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