Isolation and characterization of a thermostable cellulase-producing <Emphasis Type="Italic">Fusarium chlamydosporum</Emphasis> |
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Authors: | Yongling Qin Haiyan He Nan Li Min Ling Zhiqun Liang |
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Institution: | (1) College of Life Science & Technology, Guangxi University, 530005 Nanning, Guangxi, China;(2) Department of Chemistry & Life Science, Hechi University, 546300 Yizhou, Guangxi, China |
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Abstract: | A thermostable cellulase-producing fungus, HML 0278, was identified as Fusarium chlamydosporum by morphological characteristics and ITS rDNA sequence analysis. HML 0278 produced extracellular cellulases in solid-state
fermentation using sugar cane bassage as the carbon source. Native-PAGE analysis demonstrated that this fungus strain was
capable of producing the three major components of cellulases and xylanase, with a yield of 281.8 IU/g for CMCase, 182.4 IU/g
for cellobiohydrolase, 135.2 IU/g for β-glucosidase, 95.2 IU/g for filter paper activity, and 4,720 IU/g for xylanase. More
importantly, the CMCase and β-glucosidase produced by HML 0278 showed stable enzymatic activities within pH 4–9 and pH 4–10,
and at temperatures below 70 and 60°C, respectively. |
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